The DNA fragments of the intronic region of the CHDZ/CHDW alleles of the Hangnest Tody-Tyrant individuals captured in the Costa do Sol State Park, in southeastern Brazil. The fragments were amplified by Polymerase Chain Reaction (PCR) and electrophoresed in 2.5% agarose gel. The first column on the left (L) is a 50 bp DNA ladder, followed by the CHDZ/CHDW amplicons of the females, which present two bands (CHDZ = ~360 bp and CHDW = 400 bp), while males had only one band (CHDZ), with the negative control (N), consisting of only water.