New combination and redescription of Bumba humile, description of four new species and new records from Brazil (Araneae: Theraphosidae: Theraphosinae)

The taxonomic history of Bumba Pérez-Miles, Bonaldo & Miglio, 2014 is mainly based on the inclusion of the new species. Bumba have been characterized by the type IV urticating setae present, retrolateral process on male palpal tibia, palpal bulb resting in a ventral distal excavation of palpal tibia, metatarsus I passes between the two branches of tibial apophysis when flexed, presence of spiniform setae on prolateral and retrolateral sides of maxillae and coxae I-IV. In this paper we include the row of teeth (denticulate row) in the median region of the inferior prolateral keel in all male palps. This structure range from a residual tooth to a ridge of up to five teeth. Both, the denticulate row and the retrolateral process on male palpal tibia in males could be considered as putative synapomorphies for Bumba. Here, Homoeomma humile Vellard, 1924 is transferred to Bumba and redescribed, while the female is described for the first time. Bumba cabocla (Pérez-Miles, 2000) is synonymyzed with B. horrida (Schmidt, 1994). Bumba pulcherrimaklaasi (Schmidt, 1991) is transferred to Cyclosternum Ausserer, 1871. Four new species are described and illustrated: Bumba tapajos sp. nov. from state of Pará, Bumba cuiaba sp. nov. and Bumba rondonia sp. nov., both from states of Rondônia and Mato Grosso, respectively, and Bumba mineiros sp. nov. from Paraguay and the Brazilian states of Goiás, Mato Grosso and Mato Grosso do Sul. Diagnosis of B. horrida and B. lennoni are extended and figures of this species are presented.


INTRODUCTION
were measured between joints, in dorsal view. Total body length excludes chelicerae and spinnerets. The extended focal range images composed with Leica Application Suite version 2.5.0. For scanning electron microscopy (SEM) images, body parts were dehydrated in a series of graded ethanol washes (80 to 100%), dried to critical point, mounted on metal stubs using adhesive copper tape and nail polish for fixation and covered with gold. SEM photographs were taken with a FEI Quanta 250 scanning electron microscope at the Laboratório de Biologia Celular of Instituto Butantan, São Paulo, Brazil. Spermathecae were dissected and immerse in enzyme (Ultrazyme ® ) for 72 hours for soft tissue digestion to allow observation of internal structures.

TAXONOMY
Diagnosis. Bumba species have been characterized by the following character combination: type IV urticating setae present, retrolateral process on male palpal tibia, palpal bulb resting in a ventral distal excavation of palpal tibia, metatarsus I passes between the two branches of tibial apophysis when flexed, presence of spiniform setae on prolateral and retrolateral sides of maxillae and coxae I-IV (Pérez-Miles 2000, Bertani andCarla-da-Silva 2003, Pérez-Miles et al. 2014). Here we suggest the inclusion of the row of teeth (denticulate row) in the median region of the inferior prolateral keel (Figs 2,27,36). This structure is present on all male palps, which may range from a residual tooth (Fig. 34) to a ridge of up to five teeth . Both the denticulate row and the retrolateral process on male palpal tibia could be considered as putative synapomorphies for this genus.
Diagnosis. Males of Bumba humile can be distinguished from the other species by the very high number of labial cus-pules, over 200 ( Fig. 1). Bumba humile resembles B. tapajos due to the presence of teeth (DR) on PI ( Fig. 10) but the same are less evident, the embolus tapers towards the apex (Fig. 10) and retrolateral branch of tibial apophysis without apical spine (Fig. 9). Bumba humile females can be distinguished from the others by strongly inclined necks and reniform distal lobes (Figs 13−14).
Etymology. The species epithet is a noun in apposition taken from the Brazilian state of Rondônia.  (Fig. 27) and the embolus is shorter than that of B. rondonia sp. nov. Females could be distinguished from Bumba rondonia sp. nov. by the curved necks of the seminal receptacles (Fig. 28).
Etymology. The species epithet is a noun in apposition taken from the type locality Mineiros, in the state of Goiás. Diagnosis. Bumba cuiaba sp. nov. could be distinguished from all other species by the black longitudinal band of abdomen in males and females (Fig. 32). Males of Bumba cuiaba sp. nov. resemble B. rondonia sp. nov. and B. mineiros sp. nov. due to the curved embolus, but have shorter embolus and vestigial PS and PI (Figs 30−32, 34). Therefore, it resembles B. horrida due to the presence of a subapical spine on the retrolateral branch of tibial apophysis (Fig. 33), but can be distinguished by the slender embolus (Fig. 34). Females could be distinguished from the other species by the spermathecae with seminal receptacles with very short necks and two trilobed lobes on apex (Fig. 35).
Etymology. Species epithet is a noun in apposition taken from the city of Cuiabá, from the State of Mato Grosso, Brazil. Diagnosis. Bumba tapajos sp. nov. resembles B. humile due to the presence of a denticulate row (DR) on middle region of PI (Figs 36−40) but this is more evident and tibial apophysis with a subapical spine on retrolateral branch (Figs 41−42) absent in B. humile.
Bumba horrida   Synonymy. Recently we received larger number of couples of the Bumba from Ilha de Maracá, the type locality of B. cabocla. We observed males measuring only 25 mm until 45 mm, and females at 50 mm. Also the number of labial cuspules present variation from 0-20. Comparing details of the tip of embolus and tibial apophysis of the male palp bulb and female receptacles of the spermathecae of these specimens with those presented by Bertani and Carla-da-Silva (2003, figs 1-4;6-9), these are quiet indistinct. The female presented by   fig. 7) it is rather an immature specimen, as shown in fig. 8F. So, we proposed here the synonymy of these species.
Diagnosis. Bumba horrida differs from Bumba humile by the having fewer labial cuspules, between 0-20, male palpal bulb with PS expanded near the apex, presenting a subapical lobe (51)(52) and tibial apophysis with retrolateral side of the retrolateral branch with one strong spine (Fig. 50). Remarks.  described the species based on a male from the rain forest of Ecuador with no further information. Perafán and Pérez-Miles (2014) examined the specimen, which nowadays lacks the palpal organ and the authors considered that the figure of  of the palpal bulb was not clear enough to identify it. The presence of retrolateral node on the palpal tibiae, presence of urticating setae of type III and IV and besides other characters of generic seems the authors to be a Bumba and so the species is transferred. The aspect of the male palpal bulb   fig. 1) represented by a short embolus differs from species of Bumba which present a long and thin embolus (see Figs 8,[15][16][17][18]. Moreover, the presence of retrolateral nodule in the tibia of the palpus does not occur in species of the genus Bumba, but occurs in males of Cyclosternum. These characters show that this species resembles several species of Cyclosternum that occur in Ecuador which present a similar aspect of the male palp. We propose the transference of B. pulcherrimaklaasi to Cyclosternum.

ACKNOWLEDGMENTS
We wish to thank Flavio Uemori Yamamoto who help us to identify several new species of Bumba of the collection of the Instituto Butantan. Also our thanks to Amazonas Chagas who sent and donated several specimens of B. cuiaba sp. nov. to the collection of the Instituto Butantan, and to A.B. Bonaldo who sent us the paratypes of B. lennoni. The authors are very grateful to the editor Ricardo Pinto da Rocha, F. Pérez Miles and two anonymous reviewer for their careful reading of the manuscript. V. Ghirotto provided photos of B. humile in the wild and donated the specimen to the collection of the Instituto Butantan. To Ross Thomas by the English revision. Beatriz Mauricio help us with SEM images in the Centro de Microscopia Eletrônica of the Instituto Butantan. Funding was provided by CNPq grant PQ 303028/2014-9 to ADB.